Question

What is true about DNA polymerase used in PCR?

• A. It is used to ligate introduced DNA in recipient cells.
• B. It serves as selectable marker.
• C. It is isolated from a virus.
• D. It is active at high temperature.

Answer 1

Answer: (D)

It is active at high temperature.

DNA polymerase enzyme is an essential component for PCR due to its key role in synthesizing new DNA strands. Taq DNA polymerase ( synthesized from the thermophilic bacteria Thermus aquaticus) is the most common enzyme used for PCR amplification. This enzyme is extremely heat resistant with a half-life of 40 minutes at 95°C.

So the correct answer is option D.