The correct option is
D Middle layers lie between endothecium and tapetum
The wall of pollen sac comprises of four layers: Epidermis, Endothecium, Middle layers and Tapetum. Epidermis is followed by a single layer of Endothecium, 2-3 middle layers and a single layer of Tapetum. Tapetum is the innermost layer of the pollen sac.Tapetum surrounds the sporogenous tissue completely.
Hence, the order of layers from outside to inside is:
Epidermis, Endothecium, Middle layers and Tapetum.
Therefore, middle layers lie between the endothecium and the tapetum.
So, the correct answer is D.
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