Question

what was Frederick Sanger principal for sequencing the DNA fragments using automated DNA sequencers ? what is a DNA sequencer ?

Answer 1

The Frederick Sanger method of DNA sequencing using automated DNA sequencers is based on the principle of selective incorporation of the chain-elongating inhibitors of DNA polymerase (chain-terminating dideoxynucleotides) via enzyme DNA polymerase during in vitro replication of DNA.

DNA sequencer is an automated scientific instrument in which DNA sequencing occurs automatically. This device is used to determine the order of the nitrogen bases (adenine, cytosine, guanine and thymine).