Question

What would happen if you forget to add cytokinin to the culture medium?

• A. Callus will not develop shoot buds
• B. Callus will not develop root buds
• C. Callus will stop differentiating
• D. Both A and B

Answer 1

The correct option is A Callus will not develop shoot buds

Cytokinin is a plant growth hormone that promotes cell division or cytokinesis in the roots and shoots of the plants. In plant tissue culture, ratio of auxin and cytokinin affects root and shoot formation. If auxin is more then root formation is promoted whereas if cytokinin is more then shoot formation is promoted.

Hence, if we forget to add cytokinin to the culture medium then callus will not develop shoot buds.

So, the correct answer is 'Callus will not develop shoot buds'.