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Question
When 2 to 3 drops of Benedict's reagent are added to a urine sample and heated gently, it turns yellow. This colour change indicates that.
When 2 to 3 drops of Benedict's reagent are added to a urine sample and heated gently, it turns yellow. This colour change indicates that.
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Solution
The correct option is D Urine contains 1% glucose
When reducing sugars are heated in a basic solution of Benedict's reagent containing copper sulphate enediols are formed. Endioles react with cupric ions in Benedict’s solution and reduce it to cuprous ions. Glucose (in urine) being a reducing sugar gives a positive result for this test. As Benedict's solution is heated with urine the colour changes from green to yellow to orange. Finally, a red colour is obtained. A 1% glucose concentration gives a yellow colour. Thus the correct answer is option D.
When reducing sugars are heated in a basic solution of Benedict's reagent containing copper sulphate enediols are formed. Endioles react with cupric ions in Benedict’s solution and reduce it to cuprous ions. Glucose (in urine) being a reducing sugar gives a positive result for this test. As Benedict's solution is heated with urine the colour changes from green to yellow to orange. Finally, a red colour is obtained. A 1% glucose concentration gives a yellow colour. Thus the correct answer is option D.
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