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Question

When genomic DNA is fragmented and cloned, the screening of the desired gene is done by using ?

A
Plasmid DNA
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B
DNA probes
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C
Southern blotting
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D
PCR technique
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Solution

The correct option is B DNA probes
DNA probes are short DNA sequences that are used to identify the desired gene, out of a large number of DNA fragments, carrying the complementary nucleotide sequence. PCR - technique amplify the DNA samples. The DNA fragments from gel electrophoresis are transferred from the gel to the surface of the nylon via southern blotting. Plasmid DNA serves as vector for the gene cloning. So, the correct answer is option B.

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