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Genetic Engineering

When the reco...

Question

When the recognition sequence of two different restriction endonucleases are the same, with the location of the cut within the site also being the same, they are called as

A

Neoschizomers

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B

Isoschizomers

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C

Isocaudomers

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D

Palindromes

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Solution

The correct option is B Isoschizomers
Neoschizomers are restriction enzymes that recognize the same DNA sequence but cut at different locations within the restriction sites
Examples are SmaⅠ and XmaⅠ
Recognization sequence for XmaⅠ

Recognition sequence for SmaⅠ

Isoschizomers are restriction enzymes that recognize the same DNA sequence and cut at the same location within the restriction site
Examples Sph Ⅰ and Bbu Ⅰ

Isocaudomers are restriction enzymes that recognize the different DNA sequences but produce the same ends
Examples are BamHⅠ and Sau3AⅠ

Recognition sequence for BamHⅠ
Recognition sequence for Sau3AⅠ

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Similar questions

Q. Restriction enzymes that are used in the construction of recombinant DNA are endonucleases which cut the DNA at 'specific-recognition sequence'. What would be the disadvantage if they do not cut the DNA at specific-recognition sequence?

Q. Read the following statements and select the correct ones.
(i) Same kind of sticky ends are produced when a DNA is cut by different restriction enzymes.
(ii) Exonucleases make cuts at specific positions within the DNA.
(iii) Hind II was the first restriction endonuclease to be isolated.
(iv) A bacteriophage has the ability to replicate within bacterial cells by integrating its DNA with bacterial DNA.
(v) Presence of more than one recognition site for the same restriction enzyme within the vector complicates gene cloning.

Q. Read the following statements and select the correct ones.

(i) The same kind of sticky ends are produced when DNA has been cut by different restriction enzymes.

(ii) Exonucleases make cuts at specific positions within the DNA.

(iii) Hind II was the first restriction endonuclease to be isolated.

(iv) A bacteriophage can replicate within bacterial cells by integrating its DNA with bacterial DNA.

(v) The presence of more than one recognition site for an enzyme within the vector complicates the gene cloning.

Q. Assertion :Restriction endonucleases recognize short palindromic sequence and cut at specific sites. Reason: When a restriction endonuclease acts on a palindrome, it cleaves both the strands of DNA molecule.

Q. (a) Illustrate the recognition sequence of Eco RI and mention what such sequences are called (b) How does restriction endonuclease act on a DNA molecule?

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