Question

Where is NADP reductase enzyme located in the chloroplast?
What is the role of this enzyme in proton gradient development?

Answer 1

Location of NADP reductase

NADP reductase enzyme is located on the outer side of the thylakoid membrane of the chloroplast.

Role of NADP reductase in a proton gradient

NADP reductase reduces NADP to $NADPH+H^{+}$.
● The protons released by the reduction of NADP are pumped inside the thylakoid lumen.
● As a result, the concentration of protons increases in the thylakoid lumen as compared to the outside of the thylakoid.
● This leads to the formation of the proton gradient.