Question

Which among the following are found in $pBR322$ plasmid?
$\left(1\right)$ OR$1$ site
$\left(2\right)$ Ampicillin resistance site
$\left(3\right)$ Tetracycline resistance site
$\left(4\right)$ Lac Z gene.

• A. $1$ and $2$
• B. $2,3$ and $4$
• C. $1,3$ and $4$
• D. $1,2$ and $3$

Answer 1

The correct option is D $1,2$ and $3$

The pBR322 plasmid is an E. coli vector. It was constructed in the year 1977 in the laboratory of Herbert Boyer. In pBR322, the p stands for “plasmid” and the B and R for "Bolivar" and "Rodriguez”, respectively. It is 4361 base pair long and contains ampicillin and tetracycline resistance genes. It also has an ORI site.

So, the correct answer is '1,2 and 3'.

1,and 3'.