Question

Which among the following is the first step in the R-DNA technology process?

• A. Amplification of gene by PCR
• B. Extraction of desired gene using Restriction Endonuclease
• C. Using vector to clone the desired gene
• D. Identification of desired gene by mapping the nucleotide sequence

Answer 1

The correct option is D Identification of desired gene by mapping the nucleotide sequence

The first step in the R-DNA technology process is to identify the desired gene and map the nucleotide sequence. Once we have this information, the correct restriction enzyme and vector can be determined.