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Question

Which among the following is the first step in the R-DNA technology process?

A
Amplification of gene by PCR
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B
Extraction of desired gene using Restriction Endonuclease
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C
Using vector to clone the desired gene
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D
Identification of desired gene by mapping the nucleotide sequence
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Solution

The correct option is D Identification of desired gene by mapping the nucleotide sequence
The first step in the R-DNA technology process is to identify the desired gene and map the nucleotide sequence. Once we have this information, the correct restriction enzyme and vector can be determined.

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