The correct option is D All of the above.
Koch's postulates are:
1. The microorganisms must be found in abundance in all organisms suffering from the disease, but should not be found in healthy organisms.
2. The microorganism must be isolated from a diseased organisms and grown in pure culture.
3. The cultured microorganisms should cause disease when introduced into a healthy organism.
4. The microorganism must be reisolated from the inoculated, diseased experimental host and identified as being identical to the original specific causative agent.
Thus, the correct answer is option (D), 'All of the above'.