Which conserved motif occur in Escherichia coli genes?
A
TATA box
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B
CAAT box
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C
Pribnow box
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D
None of the above
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Solution
The correct option is C Pribnow box
Promoters of RNA polymerase II have three conserved sequences which are present between 25 bp to 100 bp upstream the start site. TATA box and CAAT box are the promoter of eukaryotic RNA polymerase and, thus, are not present in E. coli which make options A and B incorrect. TATA box is located 20 bp upstream the start site and has a consensus sequence as “TATAA/TAA/T”. CAAT box is located 70-80 bp upstream the start site and has consensus sequence as “GGT/ACAATCT”. Pribnow box is a consensus sequence present 6-10 bases upstream of the start point, in almost all prokaryotic promoters. It has sequence “TATAAT” which varies from 45 to 96% among different prokaryotes.