Question

Which does not apply to the technique known as gel electrophoresis?

• A. The DNA is pulled to the positive end of the gel box.
• B. The agarose gel provides for a porous matrix.
• C. The buffer solution helps supply ions needed to conduct an electric current.
• D. The larger DNA fragments move further down the gel than the smaller one due to their heavier weight.

Answer 1

The correct option is D The larger DNA fragments move further down the gel than the smaller one due to their heavier weight.

Gel electrophoresis is a technique used to separate and analyze macromolecules like DNA, RNA and proteins based on their size and charge. In this method, agarose gel is used to provide a porous matrix through which macromolecules will pass. Buffer solution is used to supply ions needed to conduct an electric current. The DNA is kept near the negative end of the gel box. After the electric current is applied, it is pulled to the positive end depending upon its size. The smaller DNA fragments move further down the gel than the larger one.

Thus, the correct answer is 'The larger DNA fragments move further down the gel than the smaller one due to their heavier weight.'