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Question
Which enzyme is required to prevent unwanted self-ligation of vector DNA molecules in recombinant DNA technology?
Which enzyme is required to prevent unwanted self-ligation of vector DNA molecules in recombinant DNA technology?
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Solution
The correct option is D Alkaline phosphatase
The alkaline phosphatase enzyme is required to prevent unwanted self-ligation of vector DNA molecules in procedures of rDNA (recombinant DNA) technology during the cloning process. Alkaline phosphatase removes the terminal 5'-phosphate group. Since ligation absolutely requires a 5'-phosphate group at the site of the nick, the vector DNA cannot be self-ligated in the absence of this group.
So, the correct option is 'Alkaline phosphatase'.
The alkaline phosphatase enzyme is required to prevent unwanted self-ligation of vector DNA molecules in procedures of rDNA (recombinant DNA) technology during the cloning process.
Alkaline phosphatase removes the terminal 5'-phosphate group. Since ligation absolutely requires a 5'-phosphate group at the site of the nick, the vector DNA cannot be self-ligated in the absence of this group.
So, the correct option is 'Alkaline phosphatase'.
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