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Question

Which is correct regarding genetically engineered insulin using Escherichia coli?

A
Difficulty in purifying the product
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B
Insulin obtained varies in chemical structure
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C
Obtained in large unlimited quantities
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D
Possibility of transmission of animal diseases
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Solution

The correct option is C Obtained in large unlimited quantities
Recombinant insulin (humulin) is a form of insulin produced using bacteria in recombinant DNA technology which is similar to human insulin.

The method of production of recombinant insulin is as follows:
  • Recombinant insulin is produced using Escherichia coli bacteria.
  • It is produced by cloning genes of human A and B peptides of insulin separately in different E.coli bacteria.
  • At the end, disulfide bonds are created in between both the newly created peptides to form functional insulin.
Recombinant insulin is produced in Escherichia coli by cloning the genes for human A and B peptides of insulin separately. Insulin produced by genetic engineering in this way can be easily isolated from the culture medium.

Recombinant insulin is produced using the bacteria Escherichia coli in large bioreactors which resulted in large quantities of the product.

Animal diseases cannot be transmitted through the insulin produced through genetic engineering using bacteria. It can be possible only through the products derived directly from the animals.

Recombinant insulin is similar to human insulin. It contains A and B peptides held together by disulfide bonds.

Hence the correct option is
b. Obtained in large unlimited quantities

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