Question

which method did Sanger developed for determination of amino acid sequences in proteins & how it works ?

Answer 1

Sanger developed a method which employed a reagent which was developed by him and is known as Sanger's reagent, for the determination of amino acid sequences in proteins. It works in the following way:
1. A reagent known as dinitrofluorobenzene was allowed to react it the amino groups of the proteins which results in the formation of acid stable dinitrophrenyl (DNP) derivative.
2. DNP protein is then treated with the acid and as a result polypeptide backbone is broken down which allows the free amino acid derivatives to become isolated. This is later compared to the standard ones.
3. Later, the individual chains are broken down using acids and their sequence is determined.
This method came to be known as Sanger's method of amino acid sequence determination.