Question

Which method is used by researchers for finding a gene when they know very little about that gene { this method results in a complete gene library, a collection of copies of DNA fragments that represent the entire genome of an organism}?

Answer 1

Dear Student,

Shotgun cloning - is a method used by researcher when very little information is available about gene.
In this method DNA is cut into numerous fragments either using restriction enzymes or by physical method.
These DNA fragments are sequenced individually by inserting these fragments into bacteria or yeast with plasmids.
This method was firstly used for bacteria Haemophilus influenzae.

Regards.