Which of the following are correctly matched? 1. Ligase - Joins short segments of DNA together 2. DNA polymerase - cuts DNA at specific site 3. Helicase breaks hydrogen bond between complementary pairs during DNA replication
A
1, 2, and 3 are correct
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B
1 and 2 are correct
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C
2 and 3 are correct
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D
1 and 3 are correct
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Solution
The correct option is D 1 and 3 are correct DNA ligase is an enzyme which can connect two strands of DNA together by forming a bond between the phosphate group of one strand and the deoxyribose group on another. So the point 1 is correct.
A restriction enzyme, restriction endonuclease is an enzyme that cleaves DNA into fragments at or near specific recognition sites. So the point 2 is incorrect.
Helicase breaks hydrogen bonds between the paired bases and unwinds the double helix ahead of the advancing DNA polymerase. So the point 3 is also correct.