Question

Which of the following are required in DNA fingerprinting?

• A. Restriction endonuclease
• B. <i>Taq</i> polymerase
• C. Oligonucleotide primers
• D. All the above

Answer 1

The correct option is D All the above

DNA fingerprinting involves the following steps-

1) Extraction of DNA

2) Digestion of DNA with restriction with endonucleases

3) Gel electrophoresis

4) Fragments of specific sizes having VNTR, multiplied by PCR technique (Taq polymerase and oligonucleotide primers are used).

5) Transfer of DNA nitrocellulose

6) Use of radiolabeled DNA probe

7) Recognition of repetitive sequences.

Hence, the correct option is (D).