Question

Which of the following can be measured by UV spectroscopy?

• A. Turbidity
• B. Bacterial growth
• C. Cell count
• D. All of the above

Answer 1

The correct option is D All of the above

UV spectroscopy (400-700nm) is a type of absorption spectroscopy in which light of ultra-violet region is absorbed by the molecule resulting in the excitation of the electrons from the ground state to higher energy state. It obeys the Beer-Lambert law that states that when a beam of monochromatic light passes through a solution of an absorbing substance, the rate of decrease of intensity of radiation with the thickness of the absorbing solution is directly proportional to the incident radiation as well as the concentration of the solution. Hence it is used to determine turbidity, bacterial growth and cell count. Thus, the correct answer is option D.