The correct option is D Length of chromonemata
A karyotype is the number and appearance of chromosomes in the nucleus of a eukaryotic cell. Karyotypes describe the number of chromosomes and what they look like under a light microscope. The factors considered while making karyotype is the length of the chromosomes, the length of its arms, the position of the centromere, banding pattern, any differences between sex chromosomes. The length of chromonemata is not taken into consideration while preparing a karyotype.
So, the correct answer is option D.