Which of the following characteristics are required in a fluorochrome used for immunofluorescence?
A
They should have a chemical group capable of forming stable compounds with protein molecules.
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B
Fluorochromes that have not been coupled should be easy to remove.
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C
Fluorescent light emitted should be powerful & should decline minimally.
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D
All of the above
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Solution
The correct option is D All of the above D. All of the above
Solution : Immunofluorescence is shown by fluorochromes which must have a chemical group capable of forming stable compounds with protein molecules. They must be easy to remove if not coupled and the light emitted by them should be powerful enough to detect.