Question

Which of the following characteristics should be possessed by the mobile phase in a chromatography technique?

• A. Mobile phase should be non-reactive towards the sample constituent and stationery phase
• B. Sample constituents should be completely miscible with the mobile phase
• C. Detector should not respond to any changes done in the mobile phase
• D. Mobile phase should be hazardous

Answer 1

Answer: (A), (B), (C)

The correct options are
A Mobile phase should be non-reactive towards the sample constituent and stationery phase
B Sample constituents should be completely miscible with the mobile phase
C Detector should not respond to any changes done in the mobile phase

Chromatography is a technique used to separate chemical mixtures.

The mobile phase in a chromatography travels all the way up or down the solid phase along with the sample dissolved in it. So, the mobile phase should be unreactive towards the sample as well as the stationery phase or else, the motive of the experiment would be useless.

The sample must completely dissolve or mix properly with the mobile phase, or else, it won't travel through the solid phase.

The detector is supposed to observe the changes only in the chemical sample/mixture. So, the mobile phase should be such that the detector doesn't respond to any of its changes.