Question

Which of the following components are used in rDNA technology?

• A. 1, 2, 3
• B. 2, 3, 4
• C. 1, 2, 4
• D. 1, 3, 4

Answer 1

The correct option is C 1, 2, 4

rDNA technology is the process of joining two different DNA segments in a host cell and expressing this recombinant sequence.

During the process of gel electrophoresis DNA takes up sufficient amounts of Ethidium bromide (EtBr) dye. EtBr gets intercalated between the DNA bases which in presence of ultraviolet light exhibits fluorescence.

Agarose gel electrophoresis (AGE) is a process of separating a mixture of charged molecules like DNA under the influence of electric current. When the mixture containing DNA is subjected to electrophoresis, DNA fragments move towards the positive electrode as DNA is negatively charged and they get seperated on the basis of their molecular weights.

Restriction endonucleases cause a cut on DNA segments at a specific sequence. For example, EcoRI cleaves the DNA double helices into fragments at specific sequence GAATTC, and cuts both DNA strands between the G and the A nucleotides.