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Question

Which of the following contributes to change in DNA's buoyant density?

A
Operon
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B
Intergenic sequence
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C
Base composition
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D
Cell size
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Solution

The correct option is C Base composition
DNA density is directly proportional to the percentage of GC(Guanine and cytosine) content in the genome. From density gradient centrifugation we know that the smaller peak is because of the base composition difference from the genome average, which allows it to form a separate fraction by virtue of its distinct buoyant density.

An operon is a genetic unit which consists of one or more structural genes encoding the polypeptide, and an adjacent operator gene that regulates the transcriptional activity of the structural gene.

Intergenic DNA includes any DNA that is 5′ or 3′ that contains elements of transcribed untranslated DNA.

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