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Question

Which of the following device can be used to measure the bacterial growth on the basis of its turbidity?

A
Centrifuge
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B
Spectrophotometer
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C
Colorimeter
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D
None of the above
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Solution

The correct option is B Spectrophotometer
The spectrophotometer measures the turbidity or optical density. Turbidity is the measure of the amount of light absorbed by a bacterial suspension. The degree of turbidity in the broth culture is directly proportional to the number of microorganism present i.e. microbial density. Increasing turbidity of the broth medium indicates an increase in the microbial cell mass and a decrease in cell mass is seen by a decrease in turbidity. Hence death phase will have the least optical density. Thus the correct answer is option B.
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