The correct option is D Increased thickening of inner wall of guard cells
During opening of stomata, accumulation of K+ ions in guard cells occurs. Due to this, there is creation of water potential gradient between guard cell and subsidiary cells. Water moves inside the guard cells and increases the turgor pressure of guard cells. When turgidity increases within the two guard cells flanking each stomatal aperture or pore, the thin outer walls bulge out and force the inner walls into a crescent shape making pores between the guard cells. But there is no increased thickening of inner wall of guard cells.