The correct option is D Increased thickening of inner wall of guard cell
The opening and closing of stomata in governed by increase or decrease of solutes in the guard cell, which cause them to take up or lose water respectively. Accumulation of K+ ions in guard cell, increase of turgor pressure of guard cell and creation of water potential gradient between guard cell and subsidiary cell are some of the events occur in opening and closing of stomata. No change is observed in the thickening of inner wall of guard cell during opening and closing of stomata.