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Question
Which of the following enzyme creates a homonucleotide overhang at the 3′-OH end of the DNA? It can also make blunt ends sticky by adding poly AA and poly TT.
Which of the following enzyme creates a homonucleotide overhang at the 3′-OH end of the DNA? It can also make blunt ends sticky by adding poly AA and poly TT.
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Solution
The correct option is A Terminal Transferase
- Blunt ends of the end are the simple ends of DNA which are non cohesive, the blunt ends terminate in a base pair so they can't be further extended.
- While Terminal transferase are non template enzymes which catalyze the reaction of addition of nucleotide at the 3' prime end of the DNA polymer.
- They are also used in the PCR technique to add Adenine and Thymine to the overhang of the DNA strand to create a sticky end so that a circular DNA can be formed.
- Adenine and thymine form a base pair and that is why they are used to create sticky ends by the enzyme
- Therefore the answer option A is correct.
- Blunt ends of the end are the simple ends of DNA which are non cohesive, the blunt ends terminate in a base pair so they can't be further extended.
- While Terminal transferase are non template enzymes which catalyze the reaction of addition of nucleotide at the 3' prime end of the DNA polymer.
- They are also used in the PCR technique to add Adenine and Thymine to the overhang of the DNA strand to create a sticky end so that a circular DNA can be formed.
- Adenine and thymine form a base pair and that is why they are used to create sticky ends by the enzyme
- Therefore the answer option A is correct.
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