Question

Which of the following enzyme/s is used to obtain protoplast from a parent plant cell during somatic hybridization?

• A. Colchicine
• B. Pectinase
• C. Cellulase
• D. Both B and C

Answer 1

The correct option is D Both B and C

A plant cell consists of a cell wall covering its protoplasm. So as to obtain protoplasm during somatic hybridization, cell wall has to be removed. The cell wall is composed of cellulose and pectin. So the application of pectinase and cellulose will dissolve or digest pectin and cellulose respectively leaving protoplasm.

So, the correct option is 'Both B and C'.