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Question
Which of the following enzyme was first isolated and purified in the form of crystals __________.
Which of the following enzyme was first isolated and purified in the form of crystals __________.
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Solution
The correct option is B Urease
In 1897, Eduard Buchner discovered that yeast extracts could ferment sugar to alcohol, proving that fermentation was promoted by molecules that continued to function when removed from cells. Frederick W. Kuhne called these molecules like enzymes. The isolation and crystallization of urease by James Sumner, in 1926, provided a breakthrough in early enzyme studies. Sumner found that urease crystals consisted entirely of protein, and he postulated that all enzymes are proteins. In the absence of other examples, this idea remained controversial for some time. Only in the 1930s, was Sumners conclusion widely accepted, after John Northrop and Moses Kunitz crystallized pepsin, trypsin, and other digestive enzymes and found them also to be proteins.
In 1897, Eduard Buchner discovered that yeast extracts could ferment sugar to alcohol, proving that fermentation was promoted by molecules that continued to function when removed from cells. Frederick W. Kuhne called these molecules like enzymes. The isolation and crystallization of urease by James Sumner, in 1926, provided a breakthrough in early enzyme studies.
Sumner found that urease crystals consisted entirely of protein, and he postulated that all enzymes are proteins. In the absence of other examples, this idea remained controversial for some time.
Only in the 1930s, was Sumners conclusion widely accepted, after John Northrop and Moses Kunitz crystallized pepsin, trypsin, and other digestive enzymes and found them also to be proteins.
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