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Question

Which of the following features is not necessary for cloning vectors?

A
Origin of replication
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B
High copy number
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C
Selectable marker
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D
Cloning sites
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Solution

The correct option is B High copy number
The features that are required to facilitate cloning into a vector are origin of replication, selectable marker and cloning sites. The sequence of ori is also responsible for controlling the copy number of the linked DNA. So, if one wants to recover many copies of the target DNA it should be cloned in a vector whose origin supports a high copy number.

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