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Process of DNA Replication

Which of the ...

Question

Which of the following features is required to facilitate cloning into a vector?

A

Origin of replication

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B

Selectable marker

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C

Cloning sites

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D

All of these

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Solution

The correct option is D All of these
The ori site, which is the origin of replication, a selectable marker region which codes for resistance to an antibiotic, and cloning sites are all required to facilitate cloning into a vector.

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Similar questions

Q. Which of the following features are required to facilitate cloning into a vector?
a. Origin of replication (ori)
b. Selectable marker
c. Cloning site

Q. Which of the following are essential features of a cloning vector used to create transgenic organisms:
1.Presence of ori site
2.Presence of selectable markers
3.Presence of restriction endonuclease recognition sites

How to facilitate cloning into a vector

Q. Which among the following are features required in a vector to facilitate cloning?
I. It should have ori - a sequence from where replication starts.
II. It should have a selectable marker.
III. It should have several recognition sites for a particular restriction enzyme.
IV. It should have a single recognition site for commonly used restriction enzymes.

Q. Read the given statements and select the correct option.
Statement 1 : The cloning vector is required to have very few, preferably single, recognition sites for the commonly used restriction enzymes.Statement 2 : Presence of more than one recognition sites within a cloning vector will generate several fragments, which will complicate the process of gene cloning.

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