Which of the following is a method of covalent bonding used during immobilisation?
A
Diazotization
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B
Phosphorylation
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C
Dehydrogenation
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D
None of the above
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Solution
The correct option is A Diazotization
Covalent bonding is one of the commonly used methods of enzyme immobilization which engages the formation of covalent bonds between the enzymes & the support. Diazotation is a method of convalent bonding which displays the bonding between the amino group of support & tyrosyl or histidyl group of enzyme.