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Question

Which of the following is a restriction endonuclease?

A
DNaseI
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B
HindIII
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C
RNase
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D
Protease
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Solution

The correct option is B HindIII
A restriction endonuclease is an enzyme that cleaves DNA into fragments at or near specific recognition sites within molecules known as restriction sites. Hind III is a restriction endonuclease.

DNase I - is a nuclease that cleaves DNA preferentially at phosphodiester linkages adjacent to a pyrimidine nucleotide, yielding 5'-phosphate-terminated polynucleotides with a free hydroxyl group on position 3', on average producing tetranucleotides.
RNase - Cleaves RNA molecules
Protease - an enzyme that breaks down proteins into smaller polypeptides or single amino acids.

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