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Question

Which of the following is correct about nucleases?

A
It is a large class of enzymes consisting of exonucleases and endonucleases
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B
Exonucleases remove nucleotide from only one end of the DNA
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C
Endonucleases make specific cuts within the DNA by breaking ionic bonds
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D
All of them recognise palindromic nucleotide sequences in the DNA
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Solution

The correct option is A It is a large class of enzymes consisting of exonucleases and endonucleases
Nucleases are a large class of enzymes that degrade DNA by breaking phosphodiester bonds between the nucleotides. Based on its site of action on DNA, they are divided into two types:

Exonuclease
  • It removes nucleotides by breaking the phosphodiester bond
  • It removes only one nucleotide at a time from either end of a DNA
  • It is present in both prokaryotes and eukaryotes
Endonucleases
  • It recognizes palindromic nucleotide sequences within the DNA molecule called recognition site and breaks phosphodiester bond at a particular nucleotide within the restriction site.
  • It is present in prokaryotes and provides protection against invading bacteriophage DNA

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