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Question

Which of the following is correctly matched?

A
Agrobacterium tumefaciens - Tumour
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B
Thermus aquaticus - Bt gene
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C
pBR322 - Enzyme
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D
Ligase - Molecular scissors
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E
Hind II - Plasmid vector
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Solution

The correct option is A Agrobacterium tumefaciens - Tumour
Agrobacterium tumefaciens is the causal agent of crown gall disease (the formation of tumour) in over 140 species of dicots. It is a rod-shaped, Gram-negative soil bacterium (Smith, et.al, 1907).
Symptoms are caused by insertion of a small segment of DNA, known as T-DNA (transfer DNA) into the plant cell, which is incorporated at a semi-random location into the plant genome.
The correct matches of the other options are :
Thermus aquaticus - Taq DNA polymerase - used in PCR
pBR322 - Plasmid
Ligase - a ligase is an enzyme that can catalyze the joining of two large molecules by forming a new chemical bond,
Hind II - Restriction enzyme

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