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Question
Which of the following is employed to fragment DNA in finger printing?
Which of the following is employed to fragment DNA in finger printing?
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Solution
The correct option is B Restriction endonuclease
Enzymes that cut DNA at specific sites are called as restriction endonucleases because they cut the bonds in the middle of the polynucleotide chain. Most restriction enzymes make a staggered cut in the two strands, forming sticky ends. Restriction enzymes are highly specific, and will only cut DNA at specific base sequences, 4-8 base pairs long. Restriction enzymes are produced naturally by bacteria as a defense against viruses (they restrict viral growth), but they are enormously useful in genetic engineering for cutting DNA at precise places (molecular scissors). Short lengths of DNA cut out by restriction enzymes are called restriction fragments. Restriction enzymes are named after the bacteria species they came from, so EcoR1 is from E. coli strain R.Therefore, the correct answer is option C.
Enzymes that cut DNA at specific sites are called as restriction endonucleases because they cut the bonds in the middle of the polynucleotide chain. Most restriction enzymes make a staggered cut in the two strands, forming sticky ends. Restriction enzymes are highly specific, and will only cut DNA at specific base sequences, 4-8 base pairs long. Restriction enzymes are produced naturally by bacteria as a defense against viruses (they restrict viral growth), but they are enormously useful in genetic engineering for cutting DNA at precise places (molecular scissors). Short lengths of DNA cut out by restriction enzymes are called restriction fragments. Restriction enzymes are named after the bacteria species they came from, so EcoR1 is from E. coli strain R.
Therefore, the correct answer is option C.
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