The correct option is B Reproductive isolation
Speciation is the formation of new and distinct species in the course of evolution.
Small and random genetic changes or mutations are created within a population' gene pool all the time and the furthering of these by the next generation, are regulated by natural selection as the main driving force or mechanism of evolution. These changes accumulate over long periods in a population, to a point that sexual isolation from the rest of the population happens, and this leads to speciation.
Seasonal isolation - any variation in seasons that effectively keeps two populations of organisms from interbreeding.
Behavioural isolation - is an important evolutionary mechanism that helps members of the same species identify each other as proper mates.
Tropical isolation is not a term commonly used in evolution.