Question

Which of the following is not a common procedure in tissue culture of plants?

• A. Shoot tip culture for the purpose of micropropagation
• B. Leaf, stem, and root culture for the purpose of cell suspension cultures
• C. Protoplast culture for the purpose of genetic engineering of plants
• D. Culture of hybridized mature plant cells

Answer 1

The correct option is D Culture of hybridized mature plant cells

A) Shoot tip culture may be described as the culture of terminal portion of a shoot comprising the meristem together with primordial and developing leaves and adjacent stem tissue. It is a common process used for micropropagation

B) Suspension culture is a type of culture in which single cell or small aggregates of cells mul­tiply while suspended in agitated liquid medium. It is the process done in tissue culture using plant part such as leaf, stem, roots etc.

C) Protoplasts are naked plant cells without the cell wall, but they possess plasma membrane and all other cellular components. Protoplasts of different species can be fused to generate a hybrid and this process is referred to as somatic hybridization. Genetic transformations can be achieved through genetic engineering of protoplast DNA

D) After hybridization or suspension culture, a new mature plant is produced. This plant is identical to its parent plant

So, the correct answer is 'culture of hybridized mature plant cells'