Question

Which of the following is not a feature of plasmid $pBR322$?

• A. Double stranded circular DNA
• B. Autonomous replication
• C. Integrates with chromosomal DNA in host cell
• D. Absence of histones

Answer 1

The correct option is A Double stranded circular DNA

pBR322 is an important cloning factor which adds the DNA to the host cell. The pbr322 vector is a cloning vector for E.coli and it contains restriction sites for many restriction endonucleases such as Bam HI and EcoRI. This also has various cloning sites as well as selectable markers. These vectors are generally single-stranded DNA molecules and they have the origin of replication sites which enables the replication of any piece of DNA when this is linked to the host cell.

So, the correct option is 'Double stranded circular DNA'.