Which of the following is not a marker in pBR322?

Methicillin resistance marker

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Ampicillin resistance marker

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Tetracycline resistance marker

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None of the above

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Solution

The correct option is A Methicillin resistance marker
Methicillin is a very powerful antibiotic. Methicillin resistance in a bacteria is a dangerous scenario. It is used to treat bacterial infections caused by highly antibiotic resistant bacteria. Therefore, the use of methicillin resistance gene as markers for biotechnology is avoided as much as possible. pBR322 has ampicillin and tetracycline resistance genes that are used as selection markers.

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