The correct option is A the conformation of the polypeptide backbone
The primary structure of the protein is the sequence of the amino acids, which are linked by covalent bonds, disulphide linkages, and the amide or peptide linkages are formed between the amino and the carboxyl groups of the adjacent amino acids. The conformation of the polypeptide backbone is determined by the phi( N- Calpha) and the psi (C alpha- C) angles.