The correct option is C Single-stranded
Plasmids are autonomously replicating circular extrachromosomal double-stranded DNA found naturally in bacteria and are physically separated from chromosomal DNA. They are used as vectors to carry the gene of interest into the host cell in genetic engineering.
Plasmids are capable of replicating autonomously due to the presence of an ori site (origin of replication). Origin of replication is a sequence of DNA from where replication starts and any piece of DNA, when linked to this sequence, can be made to replicate within the host cells.
Plasmids are not single-stranded.