Question

Which of the following is not an application of PCR (Polymerase Chain Reaction)?

• A. Detection of gene mutation
• B. Molecular diagnosis
• C. Gene amplification
• D. Purification of isolated protein

Answer 1

The correct option is D Purification of isolated protein

PCR stands for Polymerase Chain Reaction. It is a technique used to obtain multiple copies of the gene (or DNA) of interest is synthesised in vitro.

It can be used for the following purposes:
1. Gene amplification - Multiple copies of the desired DNA segment can be obtained in a very short duration of time.
2. Detection of gene mutation - It may be used to detect any possible mutations in DNA.
3. Molecular diagnosis - It is used to detect HIV in suspected AIDS patients.

Proteins cannot be purified by PCR.