Question

Which of the following is not required for any of the techniques of DNA fingerprinting available at present?

• A. Polymerase chain reaction
• B. Zinc finger analysis
• C. Restriction enzymes
• D. DNA - DNA hybridization

Answer 1

The correct option is B Zinc finger analysis

Zinc finger is a small protein structural motif that is characterised by the coordination of one or more zinc ions in order to stabilise the protein fold.
Zinc finger analysis is not required for any of the techniques of DNA fingerprinting available at present.

DNA fingerprinting is a chemical test that shows the genetic makeup of a person or other living things.
The technique of DNA fingerprinting includes :
i) Isolation of DNA
ii) Polymerase chain reaction may be performed if the DNA obtained from a crime site might be too small
ii) Digestion of DNA by restriction endonucleases
iii) Separation of DNA fragments through gel electrophoresis
iv) Transfering of separated DNA fragments to synthetic membrane
v) Hybridisation using labelled VNTR probe (DNA -DNA hybridisation)
vi) Detection of hybridised DNA fragments by autoradiography
vii) Interpretation of band patterns of different individuals.