Question

Which of the following is not required for any of the techniques of DNA fingerprinting available at present?

• A. Polymerase chain reaction
• B. Zinc finger analysis
• C. Restriction enzymes
• D. DNA - DNA hybridisation

Answer 1

The correct option is B Zinc finger analysis

A. Polymerase chain reaction is used to amplify DNA into large amounts.

B. Zinc finger is a kind of structural motif that is characterized by coordination between zinc ions to stabilize the tertiary structure of proteins.

C. Restriction enzymes are the endonucleases that make a cut at specific positions within the DNA.

D. DNA-DNA hybridisation is a technique of molecular biology which is used to measure the similarity between DNA sequences.

Hence, zinc finger analysis which is related to proteins, is not required for DNA fingerprinting. So, the correct answer is 'Zinc finger analysis'.