Question

Which of the following is not required in PCR?

• A. Primers
• B. DNA template
• C. Agarose gel
• D. Taq polymerase

Answer 1

The correct option is C Agarose gel

PCR or Polymerase Chain Reaction is a technique used in molecular biology to create several copies of a certain DNA segment.

For a PCR reaction, DNA template, DNA polymerase – Taq polymerase, oligonucleotide primers, deoxyribonucleotide triphosphate, buffer system are required.

But agarose gel is not required. It is used when DNA fragements have to be separated.