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Question

Which of the following is responsible for initiating replication in a plasmid?

A
Gene to be cloned
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B
Complete plasmid
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C
Origin of replication
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D
All of these
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Solution

The correct option is C Origin of replication
DNA replication refers to the process by which identical copies of DNA are synthesised within the cells. There is a specific DNA sequence called the origin of replication, which is responsible for initiating replication.

Plasmids are autonomously replicating circular extrachromosomal DNA found in certain bacteria and are physically separated from chromosomal DNA.

Plasmids are autonomously replicating as they have a specific DNA sequence known as ‘origin of replication’.

Therefore, for the multiplication of any foreign piece of DNA in an organism, it is usually linked to the plasmid. Thus, a foreign DNA (gene to be cloned) is linked with the origin of replication, so that it can replicate and multiply itself in the host organism.

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